4142 Cytokine Gene Polymorphisms in Iranian Patients with Kidney Acute Rejection

Saturday, 17 October 2015
Hall D1 Foyer (Floor 3) (Coex Convention Center)

Alireza Zarebidoki, MSc , Molecular Immunology Research Center (MIRC), Tehran University of Medical Sciences, Tehran, Iran

Mina Abrari, MSc , Department of Cell and Molecular Biology, University of Tehran, Tehran, Iran

Ali Akbar Amirzargar, PhD , Department of Immunology, Tehran University of Medical Sciences, Tehran, Iran

Background: Acute rejection (AR) in kidney transplantation is one the most important causes of rejection in Iran and the world. Considering the role of inflammatory cytokines in this process and due to this fact that genetic polymorphisms can alter the function of these cytokines, we aimed to evaluate various single nucleotide polymorphisms (SNPs) related to TNF-α, IL-6, IFN-γ and IL-1β cytokine genes.

Methods: Genomic DNA was extracted from whole blood of 56 patients with acute rejection, and 56 patients with a stable graft function (SGF). A Polymerase chain reaction with the sequence specific primers (PCR-SSP) was performed using related kits. The results were analyzed by statistical software SPSS and Epiinfo. 

Results: The frequency of A and G alleles related to -308 and -138 positions of TNF-α, and alleles C and G of the -174 position related to IL-6   showed a significant association in patients with a transplanted kidney (Both AR and SGF) compared to controls (P value<0.05).  Data related to both TNF-α SNPs, and GG, CG genotypes of -174 position (IL-6) revealed a significant relationship between AR and healthy controls. In addition, results from the comparison of SGF and healthy controls in -238(TNF-α) and -174(IL-6) positions showed a significant correlation. Haplotype analysis among study groups also displayed statistically significant associations.

Conclusion: This study found an association between TNF-α and IL-6 gene polymorphisms in kidney graft rejection or survival processes. More studies with greater samples of various populations are needed in order to confirm this finding.