Probiotic Recombination Protein Effect on Atopic Dermatitis

Recently, probiotic treatment becomes a new therapy for allergic diseases. Based on the previous research from our lab, we discovered the enzyme dehydrogenase is the major material of probiotic extract. We want to investigate what the recombinant enzyme effect on allergic disease. Then we used Dermatophagoides pteronyssinus (Der p)-induced atopic dermatitis (AD) mouse model to demonstrate the effect of probiotic recombinant protein. We found probiotic recombinant protein could decrease mouse serum total IgE and Der p-specific IgE. In the on other hands, probiotic protein also could recover the physiological function of skin, decrease Langerhans cell infiltration into epidermal and attenuate cytokine thymic stromal lymphopoietin (TSLP) production in skin tissue. Next, we postulate the recombinant protein could enhance physiological function of the anatomical barrier, the skin. We used the wound healing model of human keratinocyte, HaCaT cell and pre-treated cell with the enzyme before wound formation. The experiment determined the enzyme promote wound healed faster than untreated group. Through these results, we may explore the effect and mechanisms of recombination dehydrogenase in Der P.-induced AD model.