We investigate how IL-17A affect on airway smooth muscle contractility.
Methods: We made conditional knock out mice (integrin beta8 on dendritic cells). Racking of the integrin mouse fails to induce Th17 cells from abTcells. Then, we checked airway inflammation and airway reactivity after OVA challenge. And we also investigate smooth muscle contraction with or without IL-17A incubation.
Results: Knockout mouse had smaller hyperreactivity than that of wild type mouse. But evaluation of airway inflammation in both mouse were similar.
From muscle bath assay, IL-17A exaggerated smooth muscle contraction in 1 to 12hrs incubation with IL-17A.
In late phase, RhoA and ROCK2 protein expressions were elevated. On the other hand, in early phase RhoA and ROCK2 protein did not increase. But RhoA activation was occurred, in connection with PKC activation.
Conclusions: These results suggest that IL-17A directly exaggerates airway smooth muscle contractility. IL-17A induces RhoA and ROCK2 protein expression through NF-kB pathway. And the cytokine acutely activates RhoA through a PKC-alpha dependent pathway. We also speculated that IL-17A might activate RhoA by activating PKC-alpha and p115RhoGEF.