Methods: We enrolled 80 patients with ATD-induced hepatitis and 238 ATD-tolerant subjects. DNA was isolated from whole blood and genotyped for the single nucleotide polymorphisms (SNPs) in CD28, CTLA4, CD40 and CD40LG. Genotype frequencies of SNPs and haploptyes were compared between patients with ATD-induced hepatitis and ATD-tolerant patients.
Results: In selected SNPs of CD28 (rs3116496), CTLA4 (rs5742909, rs231775, rs3087243, rs17268364), CD40 (rs1800686, rs1883832) and CD40LG (rs3092952), genotype frequencies were not different between case and control subjects. In the analysis of haplotypes of CTLA4 and CD40LG genes, there was no significant relationship with ATD-induced hepatitis.
Conclusions: These findings indicate that genetic polymorphisms of costimulatory molecules (CD28, CTLA-4, CD40 and CD40L) are not associated with the development of ATD-induced hepatitis in Korean population, and suggest that co-stimulatory molecules do not play important roles in the pathogenesis of ATD-induced hepatitis.