4135 Association between DNA hypomethylation at IL13 gene and allergic rhinitis in house dust mite-sensitized subjects.

Saturday, 17 October 2015
Hall D1 Foyer (Floor 3) (Coex Convention Center)

Jingyun Li, PhD , Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Beijing, China

Yuan Zhang, MD , Department of Otolaryngology Head and Neck Surgery, Beijing Institute of Otolaryngology, Beijing, China

Luo Zhang , Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, China

Background: Allergic rhinitis (AR) is a complex disease, in which gene-environment interactions contribute to its pathogenesis. Epigenetic modifications such as DNA methylation play an important role in the regulation of gene function. As IL13, a pleiotropic cytokine, may be important in conferring susceptibility to AR, the aim of the present work was to assess the relationship between a CpG island methylation status at the upstream of IL13gene and house dust mite (HDM)-sensitized AR in Han Chinese subjects.

Methods: A total of 60 HDM-sensitized AR patients and 65 control subjects were enrolled as two independent cohorts from Beijing and Liaoning. MassARRAY EpiTYPER was used to systematically screen the status of DNA methylation in peripheral blood leukocytes. IL13mRNA expression was measured by real-time quantitative PCR.

Results: The mean level of methylation was decreased in the AR patient-group compared with the control-group (P = 0.01). Two of a total of 33 IL13CpG units analyzed (CpG units 24:25:26 and 38:39) showed significant differences in methylation status between the AR patient-group and the control-group; with DNA hypomethylation at CpG38:39 significantly associated with higher risk of HDM-sensitized AR in both independent cohorts and a combined cohort (Beijing: OR=1.22, 95%CI=1.03–1.43, P=0.018; Liaoning: OR=1.53, 95%CI=1.06–2.38, P=0.022; combined OR=1.23, 95%CI=1.06–1.43, P=0.006). Methylation level of CpG38:39 correlated negatively with both IL13mRNA expression and serum total IgE level.

Conclusions: DNA hypomethylation of IL13 gene may be associated with increased risk of AR from HDM-sensitization.