Methods: A total of 60 HDM-sensitized AR patients and 65 control subjects were enrolled as two independent cohorts from Beijing and Liaoning. MassARRAY EpiTYPER was used to systematically screen the status of DNA methylation in peripheral blood leukocytes. IL13mRNA expression was measured by real-time quantitative PCR.
Results: The mean level of methylation was decreased in the AR patient-group compared with the control-group (P = 0.01). Two of a total of 33 IL13CpG units analyzed (CpG units 24:25:26 and 38:39) showed significant differences in methylation status between the AR patient-group and the control-group; with DNA hypomethylation at CpG38:39 significantly associated with higher risk of HDM-sensitized AR in both independent cohorts and a combined cohort (Beijing: OR=1.22, 95%CI=1.03–1.43, P=0.018; Liaoning: OR=1.53, 95%CI=1.06–2.38, P=0.022; combined OR=1.23, 95%CI=1.06–1.43, P=0.006). Methylation level of CpG38:39 correlated negatively with both IL13mRNA expression and serum total IgE level.
Conclusions: DNA hypomethylation of IL13 gene may be associated with increased risk of AR from HDM-sensitization.