The aim of the current study was to investigate 25-hydroxy vitamin D levels in children with food allergy and different variants of TaqI, BsmI and FokI VDR polymorphisms. Understanding the functional mechanism of VDR gene polymorphism may provide a strategy for prevention and treatment of food allergy.
Materials and methods: We examined 86 infants with food allergy aged from 1 to 12 months, who were on the artificial feeding. Measurement of 25[OH]D levels was performed by immunoenzyme method. RT-PCR with melting curve analysis was used for TaqI, BsmI and FokI VDR single nucleotide polymorphisms (SNP) detection. For statistical analysis Kruskal-Wallis, post-hoc and unpaired Aspin-Welch tests were used.
Results: Frequencies of allelic variants AA:AG:GG in FokI site were 0.20:0.64:0.16 respectively, as AA:AG:GG 0.23:0.48:0.29 in BsmI site and CC:TC:TT 0.23:0.42:0.35 in TakI site. Significantly decreased serum 25[OH]D levels in carriers of variants AG and GG comparably variant AA carriers in FokI site (32.95±2.118 vs 40.25±1.898 ng/mL, p=0.0188) were revealed. Statistically significant differences in serum 25[OH]D levels for TaqI and BsmI sites were not detected.
Concluson: FokI VDR polymorphism determine vitamin D status in children with food allergy.