Monday, 8 December 2014
Exhibition Hall-Poster Area (Sul America)
Maicon Paulo, MSc
,
School of Agricutural Sciences and Veterinary Medicine, Pontifícia Universidade Católica Do Paraná, São José dos Pinhais, Brazil
Fábio Nogueira
,
School of Agricutural Sciences and Veterinary Medicine, Pontifícia Universidade Católica Do Paraná, São José dos Pinhais, Brazil
Michelle Barbosa, PhD
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School of Medicine, University of São Paulo, Ribeirão Preto, Brazil
L. Karla Arruda, MD, PhD
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Department of Medicine, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Brazil
Nelson Rosario Filho, MD PhD
,
Pediatrics, Professor of Pediatrics, Federal University of Parana, Curitiba, Brazil
Marconi Rodrigues De Farias, PhD
,
School of Agricultural Sciences and Veterinary Medicine, Pontifícia Universidade Católica Do Paraná, São José dos Pinhais, Brazil
Background: Can f 1 is the major allergen of the epithelium of dogs. This is a lipocalin adhesive nature, ubiquitous, they may remain in suspension for long periods and be readily inhaled. Periodic environmental cleanup, deleting the dog sterilization and frequent baths have been recommended to reduce ambient concentrations of Can f 1, but without concrete results. Recently, modulating topical solutions of allergens have been shown to lower concentrations in the hair of these dogs and cats, however, their actual effectiveness requires scientific documentation. This study evaluate the effectiveness and power of residual Free & Clear – modulator solution of allergen composed of hydrolyzed collagen, allantoin, panthenol and aloe vera gel - (Bayer- Brazil), a modulator solution of allergens in minimizing the concentrations of Can f 1 in the fur of dogs.
Methods: Were evaluated 80 healthy dogs older than one year, regardless of size, weight and race. Of these, 40 were submitted to the use of Free & Clear (Group 1) and 40 were washed with water only (Group 2). A sample of dust present in the coat of each dog was collected before (T0), one hour after (T1) and one week after use of the solution. The same procedure was carried out in Group 2. All samples were separated into a fine powder and used to determine the levels of Can f 1 by enzyme-linked immunosorbent assay (ELISA). Using anti can f 1 (Indoor Biothecnologies). All data were analyzed by ANOVA and Bonferroni method, with significance level of 5% (p ≤ 0.05).
Results: There was no significant reduction in the average concentration of Can f 1 in the coat of dogs in one hour 1,271 μg.g-1 (p ≥ 0.05) and after seven days 1,24 μg.g-1 (p ≥ 0.05) using the solution.
Conclusions: The solution modulator Allergen Free & Clear used showed no efficacy in reducing concentrations of Can f 1 in the coat of dogs, and did not alter their concentrations in a one week interval.
