Monday, 8 December 2014
Exhibition Hall-Poster Area (Sul America)
Luciana Wancura Marcuz, MSc
,
School of Agricultural Sciences and Veterinary Medicine, Pontifícia Universidade Católica Do Paraná, São José dos Pinhais, Brazil
Marconi Rodrigues De Farias, PhD
,
School of Agricultural Sciences and Veterinary Medicine, Pontifícia Universidade Católica Do Paraná, São José dos Pinhais, Brazil
Maria Elisa Cavinato
,
School of Agricultural Sciences and Veterinary Medicine, Pontifícia Universidade Católica Do Paraná, São José dos Pinhais, Brazil
Michelle Barbosa, PhD
,
School of Medicine, University of São Paulo, Ribeirão Preto, Brazil
L. Karla Arruda, MD, PhD
,
Department of Medicine, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Brazil
Nelson Rosario Filho, MD PhD
,
Pediatrics, Professor of Pediatrics, Federal University of Parana, Curitiba, Brazil
Background: The Fel d 1 is the main allergen of the epithelium of cats, it is originated in sebaceous, anal and salivary glands, it is ubiquitous, highly adherent and molecular weight of 36 kd, which gives a high ability to remain in suspension. Often aspiration and environmental cleanup, baths and exclusion of the households cats have been appointed to minimize their concentrations. Recently, the use of modulator solutions have been suggested to reduce the concentration of allergens on fur from cats and dogs, however it is not been critically evaluated. This study evaluated the effectiveness and power of residual Free & Clear
®- modulator solution of allergen composed of hydrolyzed collagen, allantoin, panthenol and aloe vera gel - (Bayer – SP – Brazil), a modulator solution of allergens, in minimizing the concentration of Fel d 1 in the fur of cats.
Methods: Were evaluated 65 healthy cats, regardless of race, gender and weight. Among them, 35 cats were subjected to use the Free & Clear solution (Group 1) and 30 animals used water (Group 2). The samples of cat coat were collected through aspiration, before (T0), one hour later (T1) and seven days after (T2) the use of Free & Clear in the Group 1, and water in Group 2. All samples collected were sieved resulting in a fine dust used to determine levels of Fel d 1 by the method of enzyme-linked immunosorbent assay (ELISA) using anti-Fel d1 (Indoor Biotechnologies). All data were analyzed by ANOVA and Bonferroni method, with significance level adopted of 5 (p ≤ 0.05).
Results: Among the 65 cats evaluated, no meaningful changes were observed in the average concentration of Fel d 1 in the fur of cats in T0 (2,2623 µg.g-1±1,39477) (p ≥ 0.05), after an hour (2,0499 µg.g-1±0,6035) (p ≥ 0.05) and after a week (1,5916 µg.g-1±0,086735) (p ≥ 0.05) of the use of the solution compared to the use of water.
Conclusions: modulatory solution of allergens Free & Clear showed no efficacy in reducing the concentration of Fel d 1 in cat fur, and residual effect.
