Monday, 8 December 2014: 14:50 - 15:10
Exhibition Hall-Poster Area (Sul America)
Luciana Wancura Marcuz, MSc
,
School of Agricultural Sciences and Veterinary Medicine, Pontifícia Universidade Católica Do Paraná, São José dos Pinhais, Brazil
Marconi Rodrigues De Farias, PhD
,
School of Agricultural Sciences and Veterinary Medicine, Pontifícia Universidade Católica Do Paraná, São José dos Pinhais, Brazil
Maria Elisa Cavinato
,
School of Agricultural Sciences and Veterinary Medicine, Pontifícia Universidade Católica Do Paraná, São José dos Pinhais, Brazil
Michelle Barbosa, PhD
,
School of Medicine, University of São Paulo, Ribeirão Preto, Brazil
L. Karla Arruda, MD, PhD
,
Department of Medicine, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Brazil
Nelson Rosario Filho, MD PhD
,
Pediatrics, Professor of Pediatrics, Federal University of Parana, Curitiba, Brazil
Background: The allergens from cats have been identified as extrinsic factors involved in precipitation and exacerbation of rhinitis and allergic asthma in susceptible children and adults, in global indices ranging from 10 to 25%. The major allergen from the epithelium of the cats is the Fel d 1, responsible for 90% of these allergic reactions. The Fel d 1 is a secretoglobulin, character sticker, molecular weight of 36 kd, which confers a high ability to remain in suspension. The present study aimed to evaluate there is influence of gender and sexual reproductive state on concentrations of Fel d 1 the fur of cats. Methods: Were evaluated 66 healthy cats, 34 males (22 neutered and 12 wholes) and 32 females (20 neutered and 12 wholes), from households with multiple animals, regardless of race and weight. All samples collected were identified and sieved, thus resulting in a fine dust used to determine the levels of Fel d 1 of each sample by the method of enzyme-linked immunosorbent assay (ELISA) using anti-Fel d1 (Indoor Biotechnologies). All data were analyzed by ANOVA and Bonferroni method, and the estimate of the difference between the averages in relation to groups was estimated by the Student's T-test with significance level of 5% (p ≤ 0.05).
Results: All the cats which were evaluated obtained Fel d 1 on coat, having 2.35 µg.g-1 ± 1.29 of average concentration. The concentration of Fel d 1 in female cats was higher compared to males (p ≤ 0.05), and castrated animals have higher concentration of Fel d 1 in coat, when compared to non-castrated animals (p ≤ 0.05).
Conclusions: The Fel d 1 allergen is readily found in the epithelium of cats regardless of their epidemiological characteristics, but they reach higher concentrations in female cats and neutered animals.
