Tuesday, 9 December 2014
Exhibition Hall-Poster Area (Sul America)
Rosemeire Navickas Constantino-Silva, PhD
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Faculty of Medicine ABC, Laboratory of Clinical Immunology, Center of Research, Santo Andre-SP, Brazil
Juliano Cesar Barros, MD
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Department of Dermatology, Faculty of Medicine ABC, Outpatient Group of Vitiligo, Santo Andre-SP, Brazil
Rafael Aragon Cabrera, MD
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Department of Dermatology, Faculty of Medicine ABC, Outpatient Group of Vitiligo, Santo Andre-SP, Brazil
Camila Cacere, PhD
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Faculty of Medicine ABC, Laboratory of Clinical Immunology, Center of Research, Santo Andre-SP, Brazil
Natasha Ferraroni, MD, PhD
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Unit of Allergy and Immunology, Hospital De Base Do Distrito Federal, Brasilia-DF, Brazil
Anete Grumach, MD, PhD
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Dept Pediatrics and Clinical Medicine, Faculty of Medicine ABC, Outpatient Group of Recurrent Infections, Santo Andre-SP, Brazil
Carlos D'apparecida Santos Machado Filho, MD PhD
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Department of Dermatology, Faculty of Medicine ABC, Outpatient Group of Vitiligo, Santo Andre-SP, Brazil
Background: Vitiligo is a skin disorder that affects 1% to 2% of the world population, independently of ethnicity. It presents with white plaques and skin discoloration. The presence of antibodies against melanocytes confirms the autoimmune phenomena in this disease. Regarding cellular imune response in active vitiligo, it seems to be an imbalance between T cell CD8+ and CD4+, and, moreover, an altered expression of Natural Killer (NK) in periphery, although very few data are available. We evaluated the cellular immune effect (T cell expression) in peripheral blood in vitiligo patients who received antigenic stimulus (autologous graft), in comparison with patients with inactive Vitiligo who received autologous graft in comparison with patients with active Vitiligo without grafting.
Methods: Antigenic stimulus was done with autologous skin graft (Punch 3mm): substitution of vitiligo area with normal skin in group A (inactive vitiligo patients), and group B (active vitiligo patients). Group C: healthy individuals. Quantitative numbers of T lymphocytes subpopulations (CD3, CD4, CD8) and NK cells (CD16, CD56, CD94, CD158a) were determined by Flow cytometry. (CD94+ refers as an inhibitor receptor expressed in NK cells, CD158+ refers as an apoptosis receptor in NK cells).
Results: Three groups were evaluated: A) Inactive vitiligo patients engrafted (n=10); B) active vitiligo patients without engraftment (n=10) and C) healthy individuals (n=10). The evaluation was performed on days 0,+8,+30,+60 after skin engraftment. There was no difference of CD3+CD4+ among all groups. CD3+CD8+ was lower in patients with active vitiligo (p=0.003), CD94+ was lower in patients with inactive vitiligo (p=0.01), both comparing to healthy individuals. CD158+ was higher in patients with active vitiligo, although there was no statistically significance.
Conclusions: Data suggests that cytotoxic activity of NK cells may be down regulated in patients with active vitiligo.
Supported by FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo).
