Sunday, 7 December 2014
Exhibition Hall-Poster Area (Sul America)
Yong-Dae Kim, MD
,
Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University, Daegu, South Korea
Chang Hoon Bae, MD
,
Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University, Daegu, South Korea
Si-Youn Song, MD
,
Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University, Daegu, South Korea
Yoon Seok Choi, MD
,
Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University, Daegu, South Korea
Background: Leptin is a pleiotropic hormone that regulates food intake and metabolic and endocrine functions. Serum
leptin levels have been reported to be increased in patients with allergic rhinitis and
nasal polyposis; however, the explanation for this is unclear. We aimed to demonstrate the differential expression of
leptin receptors in normal human
nasal mucosa and
nasal polyps, and to elucidate the effects of
leptin on mucin gene expression in human
nasal polyp epithelial cells.
Methods: Normal ethmoid sinus mucosa was obtained from 10 subjects and used as a control; nasal polyps were obtained from 10 patients. Leptin receptor expression was analyzed using reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis. Leptin-induced expression of major respiratory mucins (MUC5AC and MUC5B) in the human nasal polyp epithelial cells was determined using RT-PCR and enzyme-linked immunosorbent assay.
Results: The leptin receptor expression was stronger in the nasal polyps than in the normal nasal mucosa. In human nasal polyp epithelial cells, leptin increased the expression of MUC5AC and MUC5B, in a dose- and time-dependent manner, at the gene and protein levels. Leptin-induced mucin expression was inhibited by the leptin receptor antagonist.
Conclusions: The increased expression of leptin receptors in nasal polyps implies leptin has a certain role in nasal polyposis. In addition, leptin appears to induce the expression of MUC5AC and MUC5B through leptin receptors in the human nasal polyp epithelial cells.
