Methods: BALB/c mice were divided into control, Derf, Ber, Ber + Anti-CD25 groups. All mice except for the control group were sensitized by an i.p. injection of Dermatophagoides farinae (Derf). After sensitization, the Ber and the Ber+ Anti-CD25 groups were treated with Ber intranasally after challenge. The Ber+ Anti-CD25 groups were injected intraperitoneally with 250μg of anti-CD25 monoclonal antibody (mAb) a day before the first challenge. Allergic symptom scores, eosinophil counts and serum Derf-specific IgE levels were measured. T-bet, GATA-3, interferon-γ (IFN-γ), interleukin (IL)-10, IL-13 and Foxp3 mRNA expression were determined by real-time polymerase chain reaction and Western blot. CD4+CD25+Foxp3+ T cells were assessed by flow cytometry.
Results: Symptom scores, serum Derf-specific IgE levels, GATA-3 mRNA levels, T-bet mRNA levels and tissue eosinophil counts were decreased in the Ber than the Derf groups (all, p < 0.05). In Ber + anti-CD25 mAb group, symptom scores, serum Derf-specific IgE levels, IL-13 mRNA levels, and IFN-γ mRNA levels were increased than control group. In Ber + anti-CD25 group, serum Derf-specific IgE levels, IL-13 mRNA levels and GATA-3 mRNA levels were increased than Ber groups. In Ber + anti-CD25 groups, serum IL-10 levels were decreased than control, Derf and Ber groups. In Ber +anti-CD25 mAb groups, Foxp3 mRNA levels were decreased than control group. In Ber group, Foxp3 mRNA levels were increased than control group. In Ber group, percentage of CD4+CD25+FOXP3 levels were increased than Derf group. In Ber + anti-CD25 mAb group, percentage of CD4+CD25+FOXP3 levels were decreased than Ber group. The percentage of CD4+CD25+Foxp3+ T cells were increased in the Ber than the Derf groups. And Foxp3 mRNA levels were increased in the Ber than control groups (all, p < 0.05).
Conclusions: In our studies, Ber significantly reduces allergic inflammation. And our studies suggest that the mechanism of Ber may be associated with CD4+CD25+Foxp3+ Treg cells possibly through not only number but also the function.