2062 Analysis of growth factors in serum and induced sputum from patients with asthma

Monday, 8 December 2014
Exhibition Hall-Poster Area (Sul America)

Hui Zou , Beijing Shijitan Hospital, Capital Medical University, Beijing, China, Beijing, China

Background:

epidermal growth factor (EGF), basic fibroblast growth factor( bFGF), AA isoform of

platelet-derived growth factor (PDGF-AA), BB isoform of platelet-derived growth factor(PDGF-BB)

and vascular endothelial growth factor (VEGF) are involved in the pathogenesis of airway

inflammation in asthma. This study investigates the relation between asthmatic phenotype and levels

of these mediators in induced sputum and serum.

Methods:

62 asthmatic patients were divided into eosinophilic and neutrophilic phenotype by

cytological classification in induced sputum; groups of FEV1/FVC%>70% and FEV1/FVC%< 70% by lung

function; mild, moderate and severe asthma by asthma severity. And the concentrations of EGF,

bFGF, PDGF-AA, PDGF-BB and VEGF in serum and sputum were measured using sandwich enzyme

immunoassays.

Results:

VEGF levels in serum and induced sputum were higher in subjects with eosinophilic

phenotype than that of neutrophilic phenotype, in patients with FEV1/FVC%< 70% than that of

patients with FEV1/FVC%>70% ,in severe asthma than that of mild and moderate asthma. There

were no differences of EGF, bFGF, PDGF-AA and PDGF-BB between various phenotypes.

Conclusions:

Increased VEGF in serum and induced sputum were found in asthmatics with

eosinophilic airway inflammation, more severe airflow limitation and asthma severity.

Key words: asthma; phenotype; EGF; bFGF; PDGF; VEGF