The purpose of the study - to study cellular and humoral immunity in young children with nosocomial pneumonia against intestinal dysbiosis.
We examined 27 infants with nosocomial pneumonia who have had a dysbiosis. 17 healthy children served as a control group.
Studied the number of CD3, CD4, CD8, CD16, CD20, CD25, CD95-cells with monoclonal antibodies LT Series («Sorbent", Moscow, Russia). Immunoglobulin levels were determined by Manchini. Phagocytosis of latex particles was determined by the method Kudryavtseva VP The level of IL-4 and IL-8 in serum were measured by ELISA ("cytokine", Russia).
We children of the patients showed improvement CD8 +, CD16 +, CD25 + (P <0.05) and decreased CD95 +-cells (P <0.05). Phagocytic activity was reduced (P <0.01). Immunosuppressive effect of the current long-term inflammation in the lungs and the gastrointestinal tract is manifested above all in regard to humoral immunity, decreased synthesis of IgA (P <0.05) and increased IgG and IgM (P <0.05) in comparison with data the control group. The level of IL-4 and IL-8 was increased (P <0.001) compared to the control group.
Chronic diseasesagainst the background of dysbiosis cause inflammatory changes in the mucous membrane. There is an increased absorption of protein molecules unsplit food, toxic waste products of opportunistic bacteria promoting sensitization walls of the gastrointestinal tract, allergic reactions in the body. This in marked activation of eosinophils, basophils, mast cells to release histamine, serotonin, leukotrienes, which aggravate existing functional disorders