Methods: The study cohort consists of 50 patients of parthenium dermatitis confirmed by patch testing, 22 follow up cases in remission and 40 age matched healthy controls. TH1 (IL-2 & IFN-γ) and TH2 (IL-4 & IL-10) cytokines were measured by ELISA. Telomerase activity was measured by telomere repeat amplification protocol by PCR-ELISA and telomere length by Telo TAGGGTelomere Length Assay Kit.
Results: The mean concentration of TH1 cytokines were increased significantly (p < 0.001) as compared to controls whereas it was decreased in case of TH2. In follow up remission cases levels of TH1 cytokines were significantly (p < 0.05) decreased but change in TH2 cytokines level were insignificant (p > 0.05) when compared with untreated cases. Significantly (p < 0.05) elevated levels of telomerase activity and reduced telomere length was observed in PBMC, CD4+ and CD8+T cells of parthenium dermatitis patients as compared to healthy individuals. In post-treatment remission cases mean telomerase activity was significantly (p < 0.05) reduced whereas change in telomere length was insignificant, as compared to pre-treatment cases.
Conclusions: The higher concentration of TH cytokines strengthens the hypothesis of chronic stimulation of T cells in this inflammatory disease. Elevated telomerase activity and reduced TRF length might support the understanding of mechanisms in pathogenesis of parthenium dermatitis that are characterized by the recruitment of T lymphocytes. The augmented telomerase activity in pre-treatment cases and reduced activity in case of remission signifies that this might be established as a potential diagnostic/prognostic marker for parthenium dermatitis in future.