Methods: Spleen cells obtained from BALB/c mice were cultured for 3 weeks in the presence of FEX, and the number of mast cells was counted with alcian blue. We also examined the influence of FEX on mast cells activation. Cultured mast cells were sensitized with OVA-specific IgE and these sensitized cells were stimulated with OVA in the presence of FEX for 4 hours. The levels of tumor necrosis factor (TNF)-α, vascular endothelial growth factor (VEGF) and keratinocyte-derived chemokine (KC) were examined by ELISA.
Results: FEX could not suppress mast cell growth from progenitor cells in spleen cell suspension, even when 500 ng/ml of the agent was added to cell cultures. On the other hand, treatment of mast cells with FEX caused suppression of factor production from mast cells by antigenic stimulation. The minimum concentration of the agent that caused significant suppression was 200 ng/ml.
Conclusions: The present results strongly suggest that FEX exerts inhibitory effects on mast activation and results in favorable modification of clinical status of pollinosis patients.