3084 Thiol-Disulfide Balance in Children with Atopic Dermatitis

Friday, 16 October 2015
Hall D1 Foyer (Floor 3) (Coex Convention Center)

Gulin Karacan , Dr Sami Ulus Children Hospital, Ankara, Turkey

Ilknur Bostanci, MD , Department of Pediatric Immunology and Allergy,, Dr. Sami Ulus Obstetrics, Children’s Health and Diseases Training and Research Hospital, Ankara, Turkey

Nazli Ercan , Dr Sami Ulus Children Hospital, Ankara, Turkey

Asuman Colak, MD , Dr Sami Ulus Children Hospital, Ankara, Turkey

Murat Alisik, MD , Ataturk Hospital, Ankara, Turkey

Gulay Basarir, PhD , Mimar Sinan University, Ankara, Turkey

Ozcan Erel , Ataturk Hospital, Ankara, Turkey

Ilknur Bostanci, MD , Department of Pediatric Immunology and Allergy,, Dr. Sami Ulus Obstetrics, Children’s Health and Diseases Training and Research Hospital, Ankara, Turkey

BACKGROUND: Dynamic thiol disulphide homeostasis status has critical roles in antioxidant protection, detoxification, signal transduction, apoptosis, regulation of enzymatic activity and cellular signaling mechanisms and transcription factors.

In this study, we evaluated thiol disulphide level in infants with atopic dermatitis.

METHOD: Atopic dermatitis patients (31) and healthy children (30), under 2 years, were included. This study was prospective cross-sectional. Eosinophilia %, IgE levels and native thiol (SH), total thiol, disulfide (SS) SS / SH, SS / total SH, SH / total SH values were measured by the method described by Erel & Neseliogl (1). SPSS 15 (Statistical Package for Social Sciences) were analyzed using (p<0.05).

RESULTS: Atopic Dermatitis group of native thiol 322 587 ± 64.59 micro mol s - 1, total thiol 365 197 ± 66.19 micro mol s - 1 and disulfide 21:30 ± 8.81 micro mol L-1, the control group of native thiol 365 483 ± 63.77 micromol s - 1, total thiol 399 587 ± 65.12 micro mol s - 1 and disulfide levels were found to be 17:05 4.99μmol l- ± 1 (respectively p=0.012, 0.047, 0.025). A negative total thiol level between eosinophils (r = -0608) (p = 0.001) and duration of breastfeeding has been shown to be positively correlated (r = +0512) (p = 0.000).

CONCLUSION: Our study showed that the thiol-disulfide balance weakened in children with Atopic Dermatitis and is seen as part of the registration to excessive levels of oxidative balance. This study is the first in the literature. The results showed that it is important in ensuring the thiol- disulfide balance of breastfeeding. Furthermore, the thiol-disulfide balance in monitoring in the children with Atopic Dermatitis can be used as serological markers.

References:

1) Erel O, Neselioglu S: A novel and automated assay for thiol / disulphide homeostasis. Clinical Biochemistry 47 (2014) 326-332