1059 Interleukin 1 Beta in Sputum of Patients with Asthma: Relation with Airway Obstruction and Neutrophilc Inflammation

Wednesday, 14 October 2015
Hall D1 Foyer (Floor 3) (Coex Convention Center)

Jae Woo Jung , Department of Internal Medicine, Chung-Ang University Hospital, Seoul, South Korea

Hyun Ji Song , Division of Allergy and Respiratory Medicine, Soonchunhyang University Bucheon Hospital, Bucheon, South Korea

Taehyeong Lee , Division of Allergy and Respiratory Medicine, Soonchunhyang University Bucheon Hospital, Bucheon, South Korea

An-Soo Jang , Division of Allergy and Respiratory Medicine, Soonchunhyang University Bucheon Hospital, Bucheon, South Korea

Jong-Sook Park, MD , Division of Allergy and Respiratory Medicine, Soonchunhyang University Bucheon Hospital, Bucheon, South Korea

Hun Soo Chang , Soonchunhyang University Bucheon Hospital, Bucheon, South Korea

Byoung Whui Choi, MD, PhD , Dept. of Internal Medicine, Chung Ang University, Seoul, South Korea

Choon-Sik Park, MD., PhD. , Department of Internal Medicine, Soonchunhyang University Hospital, Bucheon, South Korea

Background: Interleukin 1 beta (IL-1β), which is produced by inflammasome activation, involves to the various processes of chronic inflammatory diseases. Recently, although inflammsome activation is observed in chronic inflammatory airway diseases, its role in asthma has not yet been studied. The aim of the study was to investigate the relation of sputum IL-1β with inflammatory phenotypes and severity of asthma.

Methods: Hypertonic saline induced sputum was obtained from asthma in stable state (n=143) and in exacerbated state (n=48). Differential cell count of induced sputum was done. IL-1β was measured using sandwich ELISA in induced sputum. The levels were analyzed in terms of airway obstruction (FEV1%) and inflammation (neutrophil % and eosinophil % of induced sputum) and exacerbation frequency and lung function over 1 year or longer follow up.

Results: IL-1β levels were significantly correlated with the neutrophil cell counts of induced sputum (r=0.186, p=0.010), but not with initial FEV1% in total asthmatics (p>0.05). The correlations of IL-1β levels with neutrophil % (r=0.188, p=0.025) and eosinophil % (r=-0.178, p=0.033) were observed in stable asthmatics. In the exacerbation group, IL-1β levels were inversely correlated with FEV1% (r=-0.326, p=0.024). In long term follow up of stable asthmatics (n=71) over more than 1 year, IL-1β levels were correlated with the follow up FEV1/FVC (r=-0.318 p=0.007). Annual average exacerbation rate was also well correlated with the IL-1β levels in the subjects with neutrophilic inflammation (>70%) in sputum.

Conclusion: Sputum IL-1β may be related with neutrophilc inflammation. In exacerbated state and long - term follow up of asthma, sputum IL-1β may also be related with the extent of airway obstruction. These data suggest the IL-1β may participate to airway obsturction and exacerbation frequecy, especially in neutrophilic inflammation.