4138 Interleukin-22 Gene Variation in Inflammatory Bowel Disease

Saturday, 17 October 2015
Hall D1 Foyer (Floor 3) (Coex Convention Center)

Alireza Zarebidoki, MSc , Molecular Immunology Research Center (MIRC), Tehran University of Medical Sciences, Tehran, Iran

Salman Sadeghzade, MSc , Department of Cell and Molecular Biology, Azad University of Ashkezar Branch, Yazd, Iran

Nima Rezaei, MD, PhD , Department of Immunology, Tehran University of Medical Sciences, Tehran, Iran

Hournaz Hasanzadeh, MD , Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran

Background: Inflammatory bowel disease (IBD) is an inflammatory disorder of unknown pathogenesis. Recent studies showed that interleukine-22 plays an important role in inflammatory processes during the disease. The purpose of this case-control study was to explore the association between IL-22 gene polymorphism (rs2227501) and susceptibility to IBD.

Methods: 89 patients and 201 healthy individuals referred to the Namazi Hospital of Shiraz, Iran were entered in this study. Blood samples were collected and genomic DNA was extracted. Restriction fragment length polymorphisms polymerase chain reaction (PCR-RFLP) was performed, and data were analyzed using Chi-square and Bonferroni tests.

Results: The frequency of the A allele was significantly greater, and the T allele was significantly lower in patients compared to controls (P value = 0.02). In addition, there was a statistically significant relationship between AA genotype and IBD (P value = 0.01).

Conclusion: This is the first study of evaluating rs2227501 in Iranian patients with IBD. More studies are required in order to clarify the exact role of IL-22 polymorphisms in pathogenesis and susceptibility of inflammatory bowel disease.