Ji Won Lee
,
Seoul National University Medical Research Center, Seoul, South Korea
Da-Eun Park
,
Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul, South Korea
Heung Woo Park, MD, PhD
,
Internal Medicine, Seoul National University College of Medicine, Seoul, South Korea
Han-Ki Park, MD
,
Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul, South Korea
Boram Bae
,
Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, seoul, South Korea
Hyun Seung Lee
,
Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul, South Korea
Mingyu Kang, MD
,
Internal Medicine, Subdivision of Allergy, Chungbuk National University Hospital, Cheongju, South Korea
Soon-Hee Kim
,
Seoul National University Bundang Hospital, Seoul, South Korea
Woo-Jung Song, MD
,
Internal Medicine, Seoul National University College of Medicine, Seoul, South Korea
Sujeong Kim, MD
,
Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul, South Korea
Hye-Ryun Kang, MD, PhD
,
Regional Pharmacovigilance Center, Seoul National University Hospital, Seoul, South Korea
Yoon-Seok Chang, MD, PhD
,
Internal Medicine, Seoul National University College of Medicine, Seoul, South Korea
Jung-Won Park, MD, PhD
,
Division of Allergy and Immunology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, South Korea
Chang-Han Park
,
Department of Internal Medicine, Sung-Ae General Hospital, Seoul, South Korea
Suk-Il Chang
,
Department of Internal Medicine, Sung-Ae General Hospital, Seoul, South Korea
Sook-Hee Song, MD, PhD
,
Internal Medicine, Seoul Medical Center, Seoul, South Korea
Kyung-up Min, MD, PhD
,
Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul, South Korea
Sang-Heon Cho, MD, PhD
,
Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul, South Korea
Background: Immune responses to staphylococcal enterotoxins may contribute to the pathogenesis of asthma.
Objective: To investigate roles of staphylococcal enterotoxin B (SEB) in mouse asthma models
Methods: BALB/c mice were intranasally sensitized with Dermatophagoides pteronyssinus (Der p) and/or SEB to develop acute asthma models. Mice were grouped to see effects of SEB and Der p interactions during the sensitization and challenge periods. Outcomes were evaluated for methacholine airway hyperresponsiveness (AHR), lung inflammatory cells, lung cytokines, serum IgE, and in vitro Der p-stimulated splenocyte responses.
Results: Intranasal SEB exposure increased lung macrophage, lymphocyte, and neutrophilic infiltration; but SEB alone did not develop any typical asthmatic phenotypes. Effects of SEB on asthma phenotypes were the most evident when co-administered with Der p during the sensitization period, developing more AHR and lung eosinophilic inflammation on Der p challenge. Serum Der p specific IgE levels were also amplified by SEB co-sensitization. In in vitro Der p re-stimulation experiments, IL-5 and IL-13 responses were also increased in splenocytes from the mice co-sensitized by SEB and Der p.
Conclusion: The present findings indicate the roles of SEB in Der p-induced allergic asthma, particularly during the sensitization period.
