Objective: We investigated the composition of gut microbiota according to feeding method and the association with serum IgE in infants.
Materials and methods: Fecal samples were collected at 6 month from 47 infants in the COCOA birth cohort. Microbiota characterization was performed by using 16S rRNA shotgun sequencing.
Results: The species richness (alpha-diversity) was not different according to feeding method. The significant higher level of Firmicutes and lower level of Actinobacteria detected in mixed milk (breast and formula milk) feeding infants than breast milk feeding infants at phylum level. The proportion of Bifidobacterium was significantly reduced, while Clostridium_g4, Clostridium, and Clostridium_g6 were highly enriched in mixed milk feeding infant than breast milk feeding infant at genus level. And the level of Escherichiapositively correlated with the total serum IgE at age 1 year in mixed milk feeding infant, but not in breast milk feeding infant.
Conclusion: Feeding method is not affect to the diversity of gut microbiota. However it shapes the composition of specific bacteria and Escherichiaaffect to the development of atopy.
This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Science, ICT and future Planning(NRF-2014R1A2A1A10050687).