Mi-Jin Kang, MS
,
Asan Institute for Life Science, Asan Medical Center, Seoul, South Korea
Eun Lee, MD
,
Department of Pediatrics, Childhood Asthma Atopy Center, Environmental Health Center, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea
Kil Yong Choi, MS
,
Asan Institute for Life Science, University of Ulsan College of Medicine, Seoul, South Korea
Ha-Jung Kim, PhD
,
Asan Institute for Life Science.Asan Medical Center, Seoul, South Korea
Bong-Soo Kim, PhD
,
Department of Life Sciences, Hallym University, Chuncheon, South Korea
Song-I Yang, MD
,
Asan Medical Center, seoul, South Korea
Young-Ho Jung, MD
,
Department of Pediatrics, Bundang CHA Medical Center, CHA University School of Medicine, Seongnam, South Korea
Hyun-Ju Cho, MD
,
Department of Pediatrics, Childhood Asthma Atopy Center, Enviromental Health Center, University of Ulsan College of Medicine,, Asan Medical Center, Seoul, South Korea
Young-Ho Kim, MD
,
Department of Pediatrics, Childhood Asthma Atopy Center, Environmental Health Center, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea
Yean Jung Choi, PhD
,
Asan Medical Center, Seoul, South Korea
So-Yeon Lee, MD
,
Department of Pediatrics, Hallym University Sacred Heart Hospital, Anyang, South Korea
Yoon Hee Kim, MD
,
Department of Pediatrics and Institute of Allergy, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea
Dongin Suh, MD
,
Department of Pediatrics, Seoul National University Hospital, Seoul, South Korea
Youn Ho Shin, MD
,
Department of Pediatrics, CHA University School of Medicine, Seoul, South Korea
Kangmo Ahn, MD, PhD
,
Samsung, seoul, South Korea
Byoung-Ju Kim, MD
,
Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, OH
Hyun-Jin Kim, MD
,
Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea
Juneyoung Koh, MD
,
Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea
Soo-Jong Hong, MD, PhD
,
Department of Pediatrics, Childhood Asthma Atopy Center, Environmental Health Center, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea
Background: Bacterial colonization of the infant gut begins at birth and the gut microbiota in infant was unstable. The initial gut microbiota plays an important role in the development of immnue system. Disruption of the gut microbiota has been linked to the development of allergic diseases. Especially, feeding method has a significant impact on allergic diseases.
Objective: We investigated the composition of gut microbiota according to feeding method and the association with serum IgE in infants.
Materials and methods: Fecal samples were collected at 6 month from 47 infants in the COCOA birth cohort. Microbiota characterization was performed by using 16S rRNA shotgun sequencing.
Results: The species richness (alpha-diversity) was not different according to feeding method. The significant higher level of Firmicutes and lower level of Actinobacteria detected in mixed milk (breast and formula milk) feeding infants than breast milk feeding infants at phylum level. The proportion of Bifidobacterium was significantly reduced, while Clostridium_g4, Clostridium, and Clostridium_g6 were highly enriched in mixed milk feeding infant than breast milk feeding infant at genus level. And the level of Escherichiapositively correlated with the total serum IgE at age 1 year in mixed milk feeding infant, but not in breast milk feeding infant.
Conclusion: Feeding method is not affect to the diversity of gut microbiota. However it shapes the composition of specific bacteria and Escherichiaaffect to the development of atopy.
This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Science, ICT and future Planning(NRF-2014R1A2A1A10050687).
