METHODS: The Ig-E, Interleukin-4 (IL-4), CCL22 and interferon-g (IFNg) were examined after treatments with miRNAs in murine atopic model. In addition, atopic patient's blood samples were collected and examined for miRNA expression.
RESULTS: miR-432 reduced CCL22 chemokine gene in activated lymphocytes. In mice with a cutaneous disease similar to atopic dermatitis, Interleukin-4 was inhibited and interferon-γ was induced after treatments with miR-432. Furthermore, miR-432 levels were suppressed in the atopic patients.
CONCLUSIONS: miR-432 suppressed Th1 immune responses and induced therapeutic effects in atopic mouse model.