For the diagnosis of staphylococcal sensitization widely used immunological reactions (reaction of leucocytolis – RLL, the reaction of the inhibition of leucocytes migration – RIML, the reaction of blast transformation of lymphocytes – RBTL etc.). However, these methods are time-consuming (RIML, RBTL) or insufficiently informative (RLL).
Objective.
To develop a simple and reliable method for the diagnosis of staphylococcal sensitization in vitro.
Material and меthods.
Total surveyed 37 patients with odontogenic and urological staphylococcal infection confirmed by bacteriological analysis. The control group consisted of 10 healthy persons who have not been identified on staphylococcal infection for 6 months. Diagnostics of staphylococcus sensitization was used by authorial methodology, getting the name M-ESR. The test of blood of inspected was mixed up with a staphylococcus antigen in the standard capillary of Panchenkov and incubation 18-24 hours at a room temperature. The increase of results of reaction on 12% and higher by comparison to control of reaction of M-ESR without an antigen, diagnosed bacterial sensitization.
Results and discussion. The results of M-ESR compared with the results RIML. Positive results of M-ESR and RIML detected in 81.1% and 78.4%, respectively. In the control group, positive results were observed in 20%.
Conclusion. Proposed a simple and reliable method of laboratory diagnostics can be used for the detection of sensitization to staphylococcal infection in oral and maxillofacial surgery, otolaryngology, urology and others.