Objective: To evaluate the role of HDM derived vesicles on the development of airway inflammation, and define the origin of them.
Method: To induce the airway inflammation by HDM, 6 weeks-old mice were administrated intranasally four times with 100 μg of HDM, and then challenged intranasally 4 times on days 14, 15, 21, and 22. Lung inflammation and immunologic parameters were evaluated 48 h after the final allergen challenge and 6 h after allergen challenge on day 21, respectively. In the case of HDM derived EV administration, 6 weeks-old mice were administrated intranasally four times with 75 μg of ovalbomin (OVA) and 10 μg of HDM EV, and then challenged intranasally 4 times with 50 μg of OVA on days 14, 15, 21, and 22. Especially, polymyxyn B treatment was done simultaneously during intranasal sensitization.
Result: HDM induced Th2 dominant mixed inflammation in the airway, and it contains bacteria. In addition, vesicles was also identified from HDM, and it is possible to induce immune responses in macrophage and epithelial cell. In the side of ability of inducing inflammation, HDM derived vesicles induced airway inflammation potentially compared than free LPS and soluble portion. This immune responses are mediated LPS recognition, and the source of it is gram negative bacteria in HDM.
Conclusion: These findings show that HDM derived vesicles induced airway inflammation potentially via recognition of LPS derived from gram negative bacteria in dust mite.