Presentation: Airway Inflammation Induced By House Dust Mite Derived Vesicles Is Mainly Induced By LPS Derived from Gram Negative Bacteria in Dust Mite. ( World Allergy Congress)

Wednesday, 14 October 2015

Hall D1 Foyer (Floor 3) (Coex Convention Center)

Sang-Yoon Kim, MD , Ewha Womans University Mokdong Hospital, Seoul, South Korea

Jun-Pyo Choi, PhD , Ewha Institute of Convergence Medicine, Ewha Womans University Medical Center, Seoul, South Korea

Ji-Hyun Lee, MD , Ewha Womans University Mokdong Hospital, Seoul, South Korea

Han-Ki Park, MD , Ewha Institute of Convergence Medicine, Ewha Womans University Medical Center, Seoul, South Korea

Yoon-Keun Kim, MD, PhD , Ewha Institute of Convergence Medicine, Ewha Womans University Medical Center, Seoul, South Korea

Background: House dust mite (HDM) is well known organism as source of major allergen. Besides, HDM also possesses bacteria in their digestive system, so it is also founded feces of HDM. According to recent study, bacteria is able to secrete small circular ‘vesicles’, which is contain diverse molecules in its original bacteria. In addition, administration of these vesicles from specific bacteria induced airway inflammation and tissue destruction. In this point, we hypothesized that HDM derived vesicles play an important role on the development of airway inflammation.

Objective: To evaluate the role of HDM derived vesicles on the development of airway inflammation, and define the origin of them.

Method: To induce the airway inflammation by HDM, 6 weeks-old mice were administrated intranasally four times with 100 μg of HDM, and then challenged intranasally 4 times on days 14, 15, 21, and 22. Lung inflammation and immunologic parameters were evaluated 48 h after the final allergen challenge and 6 h after allergen challenge on day 21, respectively. In the case of HDM derived EV administration, 6 weeks-old mice were administrated intranasally four times with 75 μg of ovalbomin (OVA) and 10 μg of HDM EV, and then challenged intranasally 4 times with 50 μg of OVA on days 14, 15, 21, and 22. Especially, polymyxyn B treatment was done simultaneously during intranasal sensitization.

Result: HDM induced Th2 dominant mixed inflammation in the airway, and it contains bacteria. In addition, vesicles was also identified from HDM, and it is possible to induce immune responses in macrophage and epithelial cell. In the side of ability of inducing inflammation, HDM derived vesicles induced airway inflammation potentially compared than free LPS and soluble portion. This immune responses are mediated LPS recognition, and the source of it is gram negative bacteria in HDM.

Conclusion: These findings show that HDM derived vesicles induced airway inflammation potentially via recognition of LPS derived from gram negative bacteria in dust mite.

1134 Airway Inflammation Induced By House Dust Mite Derived Vesicles Is Mainly Induced By LPS Derived from Gram Negative Bacteria in Dust Mite.