Presentation: Bacteria Derived Extracellular Vesicles in Indoor Dust Is Closely Associated with Airway Disease and Lung Cancer: Analysis of Indoor Dust’s Microbiome and IgG Sensitization of Indoor Bacteria Derived Extracellular Vesicles. ( World Allergy Congress)

Thursday, 15 October 2015

Hall D1 Foyer (Floor 3) (Coex Convention Center)

Sae-in Kim, MD , Ewha Womans University, School of Medicine, Seoul, South Korea

Do-Yeon Kim, MD , Ewha Womans University, School of Medicine, Seoul, South Korea

Han-Ki Park, MD , Ewha Institute of Convergence Medicine, Ewha Womans University Medical Center, Seoul, South Korea

Mina Rho, PhD , Department of Computer Engineering, Hanyang University, Seoul, South Korea

Jun-Pyo Choi, PhD , Ewha Institute of Convergence Medicine, Ewha Womans University Medical Center, Seoul, South Korea

Yoon-Keun Kim, MD, PhD , Ewha Institute of Convergence Medicine, Ewha Womans University Medical Center, Seoul, South Korea

Purpose: Recent experimental evidence shows that extracellular vesicles (EVs) in indoor dust induce neurtrophilic pulmonary inflammation. In addition, IgG sensitization to indoor dust EVs appears to be a correlation for the development of asthma, COPD, and lung cancer, irrespective of cigarette smoking. In this study, we analyzed indoor dust and dust extracellular vesicles (EVs) microbiome in the apartment and hospitals. Also, we evaluated whether IgG sensitization to bacteria devrived EVs is a risk for the development of asthma, COPD, or lung cancer.

Methods: In the apartment and hospital, we collected summer and winter dust. After genomic DNA was extracted from the dust and dust EVs, 16s ribosomal DNA was amplified using the universal primer, sequenced through the next generation sequencer, and then the sequenced data was analyzed using bioinformatics. Then, Serum IgG antibody against major bacteria derived EVs in dust were measured in 90 healthy control subjects, and 294 asthma, 242 COPD, and 325 lung cancer patients

Result: Bacteria and bacteria derived EVs did not differ in diversity and community composition. Our data suggests the composition of a major dust microbiome that includes Pseudomonas, Acinetobacter, Enterococcus, and Staphylococcus. As a result of comparing the bacterial composition, Pseudomonas was dominant from apartment and summer, while Acinetobacter was dominant from hospital and winter. Especially in the winter of hospital, Acinetobacter was increased remarlably and diversity was reduced. As a result of Serum IgG antibody against major bacteria derived EVs in dust, adjusted multiple logistic regression revealed that sensitization to each bacteria derived EVs in dust were an independent risk factor for asthma, COPD and lung cancer.

Conclusion: Dust microbiome from bacteria and bacteria derived EVs were mostly composed of Pseudomonas, Acinetobacter, Enterococcus, and Staphylococcus. IgG sensitization to bacteria derived EVs of indoor dust appears to be a major risk for the development of asthma, COPD, and lung cancer.

2139 Bacteria Derived Extracellular Vesicles in Indoor Dust Is Closely Associated with Airway Disease and Lung Cancer: Analysis of Indoor Dust’s Microbiome and IgG Sensitization of Indoor Bacteria Derived Extracellular Vesicles.