The Level of Serum Interleukin 13 and Interleukin 17A and Its Effect Factors in Children with Asthma

Background

To explore the level of serum Interleukin13(IL-13) and Interleukin 17A (IL-17A) in  children with asthma.

Methods

A case-control study was performed.88 asthmatic children in asthma clinic and 28 children hospitalized in surgical for noninfectious elective surgery who came to Affiliated Children’s Hospital of Capital Institute of Pediatrics in March to December 2014 were enrolled in the study. Boys 78 and girls 38, aged 5 to 14 years old, the mean age is 7 years 5 month. The level of serum IL-13 and IL-17A were measured by the enzyme-linked immunosorbent assay (ELISA) method. The material of history, blood test, lung function test, the concentrations of fraction of exhaled nitric oxide (FeNO), vitro allergern test of asthmatic children were reserved.

Results

The level of serum IL-13 and IL-17A in asthmatics is significantly higher than control (P＜0.001). There was a positive relationship between the level of serum IL-17A and the severity of asthma (r_s=0.369，P＜0.001).The level of serum IL-17A between control and chronic persistent, control and exacerbation , chronic persistent and exacerbation are significantly different (P＜0.001). The level of serum IL-13 in allergic asthmatic and non-allergic asthmatic are significantly different (Z=-2.655，P＜0.01),but there is no difference in the level of serum IL-17A(P＞0.05). There is a positive relationship between the level of serum IL-17A and neutrophils in asthmatic children (r_s=0.216，P=0.047). There is a positive relationship between the level of serum IL-13 and sIgE of inhaled allergens (r_s=0.343，P＜0.01). There is no relationship between the level of serum IL-13 or IL-17A and lung function or FeNO. In severe asthmatic children, there is a positive relationship between the level of serum IL-13 and the level of serum IL-17A (r_s=0.395，P＜0.05).

Conclusions

The level of serum IL-13 and IL-17A of asthmatic children is higher than control. There is a positive relationship between serum IL-17A and the severity of asthmatic children. There is a positive relationship between the level of serum IL-17A and neutrophils in asthmatic children. There is a positive relationship between serum IL-13 and sIgE. In severe asthmatic children, there is a positive relationship between the level of serum IL-13 and the level of serum IL-17A.