Methods: The six-week-old BALB/c female mice were sensitized two times via intraperitoneal injection of ovalbumin (OVA) and challenged intranasally with OVA with or without different sizes (10 nm or 100 nm) of SNPs.
Results: SNPs exposure induced significant airway inflammation and AHR, particularly in the 10 nm SNPs-treated group. In OVA/SNPs asthma models, OVA with 10 nm SNPs treated group showed significant airway inflammation, more than those treated with only OVA and 100 nm SNPs-treated group. Interleukin (IL)-5, IL-6, IL-13, IL-17, Tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) levels correlated with airway inflammation in the tested models.
Conclusions: These results suggest that exposure of SNPs could enhance airway inflammation. The adjuvant effects of SNPs were found at smaller size (10 nm SNPs) in OVA treated asthma mice.