Methods: Three different strains of mice - A/J, BALB/c, and C57BL/6 – were untreated (control, each n=5) or were sensitized and challenged with ovalbumin (asthma model, each n=5). AHR was measured using methacholine and activity of GSNOR was measured in whole lung lysate.
Results: Persistent increase of AHR was observed in A/J compared to BALB/c or C57BL/6 control mice. GSNOR activity was significantly higher in A/J than BALB/c or C57BL/6 control mice (596.8±217.5, 400.0±184.8, 263.1±121.7; au, nmol/min/mg, p <0.05). In asthma model, AHR were also increased in AJ mice compared to other strains, and GSNOR activity was higher than BALB/c or C57BL/6 asthma model mice (749.7±192.5, 466.3±218.1, 474.4±24.0; au, nmol/min/mg).
Conclusion: GSNOR activity may contribute to the difference of AHR between mouse strains.