Methods : Human adipose tissue-derived stem cells (hADSCs) were cultured in DMEM/F12 medium containing 10% FBS, 10 ng/ml EGF and 2 ng/ml bFGF. A549 cells were cultured in RPMI1640 containing 10% FBS, Cells were treated for 4 hr with LPS (100 ng/ml) and P25 TiO2 nanoparticles (10 ug/ml, 50 ug/ml, 250 ug/ml) and medium as control. Cell viability was determined by MTT assay. The expression levels of IL-1a, IL-8, TNFa, and IL-10 mRNAs were determined by real-time PCR.
Results : The viability of hADSCs and A549 cells were not affected by the treatment of LPS and P25 TiO2. LPS increased the expression of IL-8 and TNFa mRNAs, but decreased the expression of IL-10 in hADSCs. P25 TiO2 increased the expression of IL-1a and IL-8 mRNAs, but decreased the expression of IL-10 in hADSCs. In A549 cells, LPS and P25 TiO2 decreased the expression of IL-1a and IL-10 mRNAs, but did not affect the expression of IL-8 and TNFa mRNAs.
Conclusion : This study shows the different inflammatory responses of hADSCs and A549 cells to different stimulating materials. The response of hADSCs to inflammatory agents are more dynamics than A549 cells, which suggests that hADSC may facilitate some manipulation in certain inflammatory situation, such as chronic airway disease.