Jung-Won Kim, MD
,
Internal Medicine, Ewha Womans University, School of Medicine, Seoul, South Korea
Yeon-Mi Kang, MD
,
Internal Medicine, Ewha Womans University, School of Medicine, Seoul, South Korea
Min-Hye Kim, MD
,
Internal Medicine, Ewha Womans University, School of Medicine, Seoul, South Korea
Mina Rho, PhD
,
Department of Computer Engineering, Hanyang University, Seoul, South Korea
Kyung-Eun Yum
,
Ewha Institute of Convergence Medicine, Ewha Womans University Medical Center, Seoul, South Korea
Hyeon-Il Choi, PhD
,
Ewha Institute of Convergence Medicine, Ewha Womans University Medical Center, Seoul, South Korea
Jun-Pyo Choi, PhD
,
Ewha Institute of Convergence Medicine, Ewha Womans University Medical Center, Seoul, South Korea
Han-Ki Park, MD
,
Ewha Institute of Convergence Medicine, Ewha Womans University Medical Center, Seoul, South Korea
Taek-Ki Min, MD, PhD
,
Pediatrics, Soonchunhyang University Hospital, Seoul, South Korea
Young Joo Cho, MD, PhD
,
Internal Medicine, Ewha Womans University, School of Medicine, Seoul, South Korea
Bok-Yang Pyun, MD, PhD.
,
Pediatrics, Soonchunhyang University, Seoul, South Korea
Yoon-Keun Kim, MD, PhD
,
Ewha Institute of Convergence Medicine, Ewha Womans University Medical Center, Seoul, South Korea
Background: Microbial infection is one of the local factors that contribute to the pathogenesis of atopic dermatitis. However, many studies had been reported the systemic effect of the microorganism, especially of the lactic acid bacteria. Until now, they only have been studying the systemic effects of local microorganisms, not the systemic microbial distribution. No study has revealed that relationship between the systemic bacteria and atopic dermatitis. Here, we performed metagenomic analysis to determine systemic bacteria distribution of atopic dermatitis patients vs. healthy control subjects.
Methods: Twenty-eight patients with atopic dermatitis and 8 healthy control subjects were enrolled. Urine was obtained in all subjects and serum was obtained in eighteen atopic dermatitis patients. After genomic DNA was extracted from the urine and serum, 16s ribosomal DNA was amplified using the universal primer, sequenced through the next generation sequencer, and then the sequenced data was analyzed using bioinformatics.
Results: The bacterial composition was nearly identical between serum and urine. However, there was notable difference of bacterial composition in the urine of the normal control and the atopic dermatitis patients. In the control group, proportion of Lactococcus, Leuconostoc, Lactobacillus, Lactobacillales(o) were significantly higher than in the patients group, and that of Alicyclobacillus, Propionibacterium, Streptophyta(o) were increased in the patients group than in the control group. Pseudomonas was commonly found in the both groups. Before treatment, Alicyclobacillus and Comamonadaceae were frequently found, however their proportion were decreased and Acinetobacter and Oxalobacteraceae(f) were increased after treatment in the urine of atopic dermatitis patients
Conclusions: We confirmed the systemic bacterial composition in the atopic dermatitis and normal controls through the metagenomic analysis of bacterial DNA in the urine and serum.
