Presentation: Roles of ADAM10 and ADAM17 in allergic rhinitis ( World Allergy Congress)

Thursday, 15 October 2015

Hall D1 Foyer (Floor 3) (Coex Convention Center)

Ji Won Seo, MD , Department of Otorhinolaryngology-Head and Neck Surgery, Dong-a University College of Medicine, Busan, South Korea

Jae Hoon Lee, MD , Department of Otorhinolaryngology-Head and Neck Surgery, Dong-a University College of Medicine, Busan, South Korea

Woo Yong Bae, MD, PhD , Department of Otorhinolaryngology-Head and Neck Surgery, Dong-a University College of Medicine, Busan, South Korea

Background: A disintegrin and metalloproteases (ADAMs) are a multi-functional gene family, they contribute to the homeostasis of the extracellular matrix, transduction of specific intracellular signals, organogenesis, inflammation, tissue remodeling, adhesion, and cell migration. ADAM17 is the best characterized of the sheddases, and its putative substrates are widespread, including various inflammatory modulators. ADAM10 is the most similar to ADAM17 in terms of protein sequence and the structural properties of their catalytic domains.

Objective: To assess the roles of ADAM 10 and ADAM 17 in allergic rhinitis by assaying for expression of these materials.

Methods: The expression of ADAM 10 and 17 was investigated in the allergic nasal mucosa compared with non-allergic nasal mucosa. Tissue samples were analyzed by real time polymerase chain reaction (PCR) and Western blotting.

Results: The ADAM17 mRNA and protein levels were significantly lower in allergic nasal mucosa than in the non-allergic nasal mucosa (P < 0.05). The ADAM10 mRNA did not differ significantly between allergic nasal mucosa and non-allergic nasal mucosa. ADAM10 protein levels were significantly higher in the non-allergic nasal mucosa than in the allergic nasal mucosa (P < 0.05).

2042 Roles of ADAM10 and ADAM17 in allergic rhinitis