Subjects and methods: Sixty AR patients with went through NPT to D.farinae. The sinus packs were placed into patients’ both nasal cavities for 5 minutes to earn nasal secretion after NPT. Total IgE, specific IgE to D. farinae, ECP, IL-8, VEGF and tryptase levels were measured by using ImmunoCAP (ThermoFisher, Uppsala, Sweden). D.farinae-specific IgE, IgA, IgG and secretory IgA antibodies were measured by ELISA. IL-8 and VEGF levels were measured by ELISA kit(Endogen, Woburn, MA and R&D Systems, Inc, Minneapolis, MN, respectively) .
Results: High levels of total IgE, specific IgE, specific IgG, specific IgA, secretory IgA as well as ECP, IL-8, VEGF and tryptase were detected in nasal secretion, but showed no significant differences between positive and negative responders. Inflammatory mediators including ECP, IL-8 and VEGF were not only detected but well correlated with specific antibodies to D.farinae ( P<0.05, respectively). Compared to ELISA method, ImmunoCAP system is more sensitive in detection of specific IgE to D.farinae. Difference between right and left nasal secretion had no statistical significance.
Conclusion: These findings confirmed the presence of specific antibodies to D.farinae-sensitive AR patients. Localized antibodies abundant in nasal mucosa may have a role in the nasal inflammation of AR patients sensitized to D.farinae.