Aim: investigate TGFβ1 levels in children with food allergy and different variants of VDR gene polymorphism.
Materials and methods: We examined 130 infants with allergic diseases aged from 1 to 12 months, who were on the artificial feeding. Measurement of TGFβ1 levels was performed by immunoenzyme method. RT-PCR with melting curve analysis was used for TaqI, BsmI and FokI VDR single nucleotide polymorphisms (SNP) detection. For statistical analysis Kruskal-Wallis and post-hoc tests were used.
Results: The frequency of allele A, homozygous A/A and heterozygous G/A genotypes in VDR gene site BsmI were significantly increased in children with allergic diseases compare population (OR = 1,81, p = 0.04; OR = 2,03, p = 0,05 and OR = 1,8, P = 0,05 respectively). Significantly decreased serum TGFβ1 levels in carriers of variants A/G and G/G comparably variant A/A carriers in FokI site (Me(q1;q3): A/A - 1134(897;1705) vs A/G – 610(511;919) vs G/G – 838(734;901) pg/mL, p=0.0216 ) were revealed. Statistically significant differences in serum TGFβ1 levels for TaqI and BsmI sites in VDR gene were not detected.
Concluson: FokI site VDR gene polymorphism may indirectly influence on TGFβ1 synthesis in allergy diseases development.