4119 Tgfβ1 level is associated with VDR gene polymorphism in children with allergy diseases

Saturday, 17 October 2015
Hall D1 Foyer (Floor 3) (Coex Convention Center)

Tatiana Sentsova , Institute of Nutrition, Moscow, Russia

Ilya Vorozhko , Institute of Nutrition, Moscow, Russia

Olga Chernyak , Institute of Nutrtion, Moscow, Russia

Vera Revyakina , Institute of Nutrtion, Moscow, Russia

Anna Timopheeva , Institute of Nutrtion, Moscow, Russia

Andrey Donnikov , DNA-Technology, JSC, Moscow, Russia

Background: vitamin D receptor (VDR) is intracellulary located on the cells of the immune system and is involved in the immune response. The development of allergic inflammation is controlled by different genes, including the gene VDR.

Aim: investigate TGFβ1 levels in children with food allergy and different variants of VDR gene polymorphism.

Materials and methods: We examined 130 infants with allergic diseases aged from 1 to 12 months, who were on the artificial feeding. Measurement of TGFβ1 levels was performed by immunoenzyme method. RT-PCR with melting curve analysis was used for TaqI, BsmI and FokI VDR single nucleotide polymorphisms (SNP) detection. For statistical analysis  Kruskal-Wallis and post-hoc tests were used.

Results: The frequency of allele A, homozygous A/A and heterozygous G/A genotypes in VDR gene site BsmI were significantly increased in children with allergic diseases compare population (OR = 1,81, p = 0.04; OR = 2,03, p = 0,05 and OR = 1,8, P = 0,05 respectively). Significantly decreased serum TGFβ1 levels in carriers of variants A/G and G/G comparably variant A/A carriers in FokI site (Me(q1;q3): A/A - 1134(897;1705) vs A/G – 610(511;919) vs G/G – 838(734;901) pg/mL, p=0.0216 ) were revealed. Statistically significant differences in serum TGFβ1 levels for TaqI and BsmI sites in VDR gene were not detected.

Concluson: FokI site VDR gene polymorphism may indirectly influence on TGFβ1 synthesis in allergy diseases  development.