Presentation: IgE reactivity of recombinant allergen pac c 3 of the Asian needle ant pachycondyla chinensis ( World Allergy Congress)

Wednesday, 14 October 2015

Hall D1 Foyer (Floor 3) (Coex Convention Center)

Kyoung Yong Jeong, PhD , Department of Internal Medicine and Institute of Allergy, Yonsei University College of Medicine, Seoul, South Korea

Myung-Hee Yi, PhD , Department of Environmental Medical Biology and Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul, South Korea

Mina Son, MSc , Department of Internal Medicine and Institute of Allergy, Yonsei University College of Medicine, Seoul, South Korea

Dongpyo Lyu, PhD , Forest Science, Sangji University, Wonju, South Korea

Jae-Hyun Lee, MD, PhD , Division of Allergy and Immunology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, South Korea

Tai-Soon Yong, MD, PhD , Department of Environmental Medical Biology and Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul, South Korea

Chein-Soo Hong, MD, PhD , Division of Allergy and Immunology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, South Korea

Jung-Won Park, MD, PhD , Division of Allergy and Immunology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, South Korea

Kyoung Yong Jeong, PhD , Derparment of Internal Medicine and Institute of Allergy, Yonsei University College of Medicine, Seoul, South Korea

Jung-Won Park, MD, PhD , Division of Allergy and Immunology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, South Korea

Background: Stings from the Asian needle ant are an important cause of anaphylaxis in East Asia. A 23-kDa protein homologous to antigen 5 is the major allergen produced by these ants. In this study, we aimed to produce a recombinant 23-kDa allergen.

Methods: Recombinant 23-kDa allergen from the Asian needle ant was expressed in Pichia pastoris and purified by ammonium sulfate precipitation and Ni-affinity chromatography. IgE reactivity was demonstrated by ELISA and immunoblotting.

Results: The recombinant protein was recognized in 5 of 6 serum samples (83.3%) from patients with demonstrated anaphylaxis to ants. IgE reactivity to a 23-kDa allergen from venom sac extract was specifically inhibited by the recombinant protein.

Conclusion: A recombinant 23-kDa allergen from the Asian needle ant was successfully produced in the methylotrophic yeast P. pastoris. This protein could be useful for the development of component-resolved diagnostics.

1159 IgE reactivity of recombinant allergen pac c 3 of the Asian needle ant pachycondyla chinensis