Presentation: Anti-allergic effect of anti-IL-33 by suppression of immunoglobulin light chain and inducible nitric oxide synthase ( World Allergy Congress)

Thursday, 15 October 2015

Hall D1 Foyer (Floor 3) (Coex Convention Center)

Tae Young Jang, MD, PhD , Department of Otorhinolaryngology, Inha University, College of Medicine, Incheon, South Korea

Chang-Shin Park, PhD , Department of Pharmacology, Inha Univ. Hospital, Incheon, South Korea

Tae-Suk Kyung, MD , Department of Otorhinolaryngology, Inha University, College of Medicine, Incheon, South Korea

Seung-Chan Yang, MD , Department of Otorhinolaryngology, Inha University, College of Medicine, Incheon, South Korea

Min-Jeong Heo, MS , Department of Otorhinolaryngology, Inha University, College of Medicine, Incheon, South Korea

Ah-Yeoun Jung, MS , Department of Otorhinolaryngology, Inha University, College of Medicine, Incheon, South Korea

Young Hyo Kim, MD, PhD , Department of Otorhinolaryngology, Inha University, College of Medicine, Incheon, South Korea

Objective: IL-33 is involved in several steps in allergic cascade. We aimed to find novel genes, which are significantly induced in allergic individuals and significantly down-regulated by anti-IL-33 treatment.

Methods: Thirty-six mice were allocated into Group A (intraperitoneally sensitized and intranasally challenged to saline), Group B (sensitized and challenged to ovalbumin), Group C (null treatment with intraperitoneal saline) and Group D (anti-IL-33 intraperitoneal injection). We checked the number of nose-scratching behaviors in 10 minutes, serum ovalbumin-specific IgE, titers of cytokines (IL-1, IL-4, IL-5, IL-10, IL-13) in bronchoalveolar lavage fluid. Using one whole lung tissue from each mouse, we performed microarray analysis and real-time PCR.

Results: Group D showed significantly reduced number of nose-scratching and lower serum ovalbumin-specific IgE. All cytokines in bronchoalveolar lavage fluid were significantly decreased after anti-IL-33 treatment. In microarray analysis, Group B (Immunoglobulin free light chain (IgFLC): 89.1 times, Nitric Oxide Synthase 2 (NOS2): 11.5 times) and Group C (IgFLC: 141.6 times, NOS2: 11.7 times) had significantly increased expression of IgFLC and NOS2 gene compared to Group A. After anti-IL-33 treatment, Group D showed significantly decreased expression (IgFLC: 49.3 times, NOS2: 6.5 times). In real-time PCR, Group B (IgFLC: 10.4 times, NOS2: 3.8 times) and Group C (IgFLC: 29 times, NOS2: 4.5 times) had significantly increased expression of these genes. After treatment, Group D showed significantly decreased expression (IgFLC: 5.0 times, NOS2: 2.5 times).

Conclusion: Anti-allergic effect of anti-IL-33 could be explained by suppression of IgFLC and NOS2 in a murine model of allergic rhinitis and asthma.

2026 Anti-allergic effect of anti-IL-33 by suppression of immunoglobulin light chain and inducible nitric oxide synthase