Effect of multiwalled carbon nanotube in OVA-induced asthma model
Il Seon Yun1) , Joo Young Kim2), In-Hong Choi3), Chein-Soo Hong1), Jung-Won Park1)
Division of Allergy & Immunology Department of Internal Medicine Yonsei University College of Medicine1) Institute of Allergy Department of Internal Medicine2) Department of
Background: Multiwalled carbon nanotubes (MWCNTs) have diverse physicochemical properties, but the potential health effects of MWCNTs are raised because they show similar shape to asbestos.
Objective: The purpose of this study was that intranasally administrated MWCNTs produce injury and/or fibrosis in mice with allergic lung inflammation.
Methods: We generated OVA-induced asthma model of C57BL/6 mice and intranasally administrated with MWCNTs. The effects of MWCNTs on OVA-asthma model were analyzed by airway hyperreactivity (AHR) measurement, bronchoalveolar lavage fluid (BALF) analysis, intracellular ROS production and histological analysis in lung tissue.
Results: Intranasally administrated-MWCNTs easily loaded in alveolar macrophage, but did not result in significant lung inflammation and intracellular ROS production of the OVA-induced asthma model. In histological analysis, MWCNTs exacerbated collagen deposition within the lung parenchyma of naïve mice and pre-existing allergic mice.
Conclusion: These results suggest that combined OVA sensitization and MWCNTs administration did not affected pre-existing allergic, but increased collagen deposition in lung parenchyma.